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Saccharothrix sp. PAL54, a new chloramphenicol-producing strain isolated from a Saharan soil

Aouiche, Adel and Sabaou, Nasserdine and Meklat, Atika and Zitouni, Abdelghani and Bijani, Christian and Mathieu, Florence and Lebrihi, Ahmed Saccharothrix sp. PAL54, a new chloramphenicol-producing strain isolated from a Saharan soil. (2012) World Journal of Microbiology and Biotechnology, 28 (3). 943-951. ISSN 0959-3993

(Document in English)

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Official URL: http://dx.doi.org/10.1007/s11274-011-0892-2


An actinomycete strain designated PAL54, producing an antibacterial substance, was isolated from a Saharan soil in Ghardaïa, Algeria. Morphological and chemical studies indicated that this strain belonged to the genus Saccharothrix. Analysis of the 16S rDNA sequence showed a similarity level ranging between 96.9 and 99.2% within Saccharothrix species, with S. longispora DSM 43749T, the most closely related. DNA–DNA hybridization confirmed that strain PAL54 belonged to Saccharothrix longispora. It showed very strong activity against pathogenic Gram-positive and Gram-negative bacteria responsible for nosocomial infections and resistant to multiple antibiotics. Strain PAL54 secreted the antibiotic optimally during mid-stationary and decline phases of growth. One antibacterial compound was isolated from the culture broth and purified by HPLC. The active compound was elucidated by uv-visible and NMR spectroscopy and by mass spectrometry. The results showed that this compound was a D(-)-threo chloramphenicol. This is the first report of chloramphenicol production by a Saccharothrix species.

Item Type:Article
Additional Information:Thanks to Springer Verlag editor. The original PDF can be found at World Journal of Microbiology and Biotechnology website: http://link.springer.com/article/10.1007%2Fs11274-011-0892-2
HAL Id:hal-00878422
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Ecole normale supérieure de Kouba (ALGERIA)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université Moulay Ismaïl de Meknès (MOROCCO)
Laboratory name:
Deposited On:30 Oct 2013 08:39

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