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Cloud point extraction of Δ9-tetrahydrocannabinol from cannabis resin

Ameur, Soumia and Haddou, Boumediene and Derriche, Zoubir and Canselier, Jean-Paul and Gourdon, Christophe Cloud point extraction of Δ9-tetrahydrocannabinol from cannabis resin. (2013) Analytical and Bioanalytical Chemistry, 405 (10). 3117-3123. ISSN 1618-2642

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Official URL: http://dx.doi.org/10.1007/s00216-013-6743-2


A cloud point extraction coupled with high performance liquid chromatography (HPLC/UV) method was developed for the determination of Δ9-tetrahydrocannabinol (THC) in micellar phase. The nonionic surfactant “Dowfax 20B102” was used to extract and pre-concentrate THC from cannabis resin, prior to its determination with a HPLC–UV system (diode array detector) with isocratic elution. The parameters and variables affecting the extraction were investigated. Under optimum conditions (1 wt.% Dowfax 20B102, 1 wt.% Na2SO4, T=318 K, t=30 min), this method yielded a quite satisfactory recovery rate (~81%). The limit of detection was 0.04 μgmL−1, and the relative standard deviation was less than 2 %. Compared with conventional solid–liquid extraction, this new method avoids the use of volatile organic solvents, therefore is environmentally safer.

Item Type:Article
Additional Information:Thanks to Springer Verlag. The definitive version is available at http://link.springer.com/article/10.1007%2Fs00216-013-6743-2
HAL Id:hal-00976845
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université des Sciences et de la Technologie Mohamed Boudiaf d'Oran - USTO (ALGERIA)
Laboratory name:
Deposited On:28 Oct 2013 17:35

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