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Irreducible coupling between physical and biological phenomena: overview of on-line and off-line physical measurements during high cell density cultures of yarrowia lipolytica

Kraiem, Hazar and Manon, Yannick and Anne-Archard, Dominique and Fillaudeau, Luc Irreducible coupling between physical and biological phenomena: overview of on-line and off-line physical measurements during high cell density cultures of yarrowia lipolytica. (2012) In: 8th International Conference on Renewable Resources and Biorefineries - RRB8, 4 June 2012 - 6 June 1012 (Toulouse, France).

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During cell cultures in bioreactor, micro-organism physiology closely interacts with physico-chemical parameters (gas and feed flow rates, mixing, temperature, pH, pressure). The specificity of microbial bioreactions in relation with irreducible couplings between heat and mass transfers and fluid mechanics, led into complex (three phases medium) and dynamic (auto-biocatalytic reaction) systems. Our scientific approach aims to investigate, understand and control dynamic interactions between physical and biological systems at different scales (macro, micro and molecular) for molecules, strains and/or bioprocess innovation in a white biotechnology context. Cells (concentration, shape, dimension, physiology) strongly affect physico-chemical properties of broth and the modification of these characteristics interacts with bioprocess performances (specific rates, yields…) with an improvement or, more generally, a decrease of yields. Among these properties, rheological behaviour is a strategy widely used to understand the impact of cells and the modification of bioprocess performances. Our approach rests on physical and physico-chemical on-line and off-line measurements in respect with accurate and stringent conditions imposed by cell culture strategy. This work leads to design and realise an original pilot based on a bioreactor (20L) with a derivation loop including a specific on-line rheometric device as well as additional physical and biological measurements. Y. lipolytica cultures were investigated with a control of growth rate by carbon feed within concentrations ranging from 0.1 up to 60gCDW/L. On-line and off-line measurements are discussed regarding similar or opposite tendencies along culture. If the off-line density could be correlated with cell concentration, on-line measurement exhibit opposite tendancy. On-line and off-line rheological measurements are consistent. Results are discussed in terms of size, morphology, surface properties, concentration, biological activity and compared to scientific literature. On-line rheology highlight about the Non-newtonian rheological behaviour of broths and the gap between on-line and off-line measurements.

Item Type:Conference or Workshop Item (Poster)
HAL Id:hal-04023184
Audience (conference):International conference without published proceedings
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Laboratory name:
Deposited On:17 Oct 2013 13:46

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