Mauline, Léïla and Gressier, Marie and Roques, Christine and Hammer, Peter and Ribeiro, Sidney J. L. and Caiut, José Maurício A. and Menu, Marie-Joëlle
Bifunctional silica nanoparticles for the exploration of biofilms of Pseudomonas aeruginosa.
(2013)
Biofouling, 29 (7). 775-788. ISSN 0892-7014
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(Document in English)
PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 17MB |
Official URL: http://dx.doi.org/10.1080/08927014.2013.798866
Abstract
Luminescent silica nanoparticles are frequently employed for biotechnology applications mainly because of their easy functionalization, photo-stability, and biocompatibility. Bifunctional silica nanoparticles (BSNPs) are described here as new efficient tools for investigating complex biological systems such as biofilms. Photoluminescence is brought about by the incorporation of a silylated ruthenium (II) complex. The surface properties of the silica particles were designed by reaction with amino-organosilanes, quaternary ammonium-organosilanes, carboxylate-organosilanes and hexamethyldisilazane. BSNPs were characterized extensively by DRIFT, 13C and 29Si solid state NMR, XPS, and photoluminescence. Zeta potential and contact angle measurements exhibited various surface properties (hydrophilic/hydrophobic balance and electric charge) according to the functional groups. Confocal laser scanning microscopy (CLSM) measurements showed that the spatial distribution of these nanoparticles inside a biofilm of Pseudomonas aeruginosa PAO1 depends more on their hydrophilic/hydrophobic characteristics than on their size. CLSM observations using two nanosized particles (25 and 68 nm) suggest that narrow diffusion paths exist through the extracellular polymeric substances matrix.
Item Type: | Article |
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Additional Information: | Thanks to Taylor & Francis editor. The definitive version is available at http://www.tandfonline.com The original PDF can be found at Biofouling website: http://www.tandfonline.com/doi/abs/10.1080/08927014.2013.798866#.Ul5E81BHAow |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE) Other partners > Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP / São Paulo State University (BRAZIL) |
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Deposited On: | 16 Oct 2013 08:15 |
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