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Ergosterol-to-Biomass Conversion Factors for Aquatic Hyphomycetes.

Gessner, Mark O. and Chauvet, Eric Ergosterol-to-Biomass Conversion Factors for Aquatic Hyphomycetes. (1993) Applied and Environmental Microbiology, 59 (2). 502-507. ISSN 0099-2240

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Official URL: http://aem.asm.org/content/59/2/502.abstract?sid=cd952432-fec7-46e4-98c6-ae3e39767d9b

Abstract

Fourteen strains of aquatic hyphomycete species that are common on decaying leaves in running waters were grown in liquid culture and analyzed for total ergosterol contents. Media included an aqueous extract from senescent alder leaves, a malt extract broth, and a glucose-mineral salt solution. Concentrations of ergosterol in fungal mycelium ranged from 2.3 to 11.5 mg/g of dry mass. The overall average was 5.5 mg/g. Differences among both species and growth media were highly significant but followed no systematic pattern. Stationary-phase mycelium had ergosterol contents 10 to 12% lower or higher than mycelium harvested during the growth phase, but these differences were only significant for one of four species examined. Availability of plant sterols in the growth medium had no clear effect on ergosterol concentrations in two species tested. To convert ergosterol contents determined in field samples to biomass values of aquatic hyphomycetes, a general multiplicative factor of 182 is proposed. More accurate estimates would be obtained with species-specific factors. Using these in combination with estimates of the proportion of the dominant species in a naturally established community on leaves resulted in biomass estimates that were typically 20% lower than those obtained with the general conversion factor. Improvements of estimates with species-specific factors may be limited, however, by intraspecific variability in fungal ergosterol content.

Item Type:Article
Additional Information:Thanks to American Society for Microbiology editor. The definitive version is available at http://aem.asm.org/content/59/2/502.abstract?sid=cd952432-fec7-46e4-98c6-ae3e39767d9b
HAL Id:hal-01494940
Audience (journal):International peer-reviewed journal
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Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
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Deposited By: Eric Chauvet
Deposited On:11 Feb 2014 10:45

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