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Study on differential expression of 1-aminocyclopropane-1-carboxylic acid oxidase genes in table grape cv. Thompson Seedless

Munoz-Robredo, Pablo and Gudenschwager, Orianne and Chervin, Christian and Campos-Vargas, Reinaldo and González-Agüero, Mauricio and Defilippi, Bruno G. Study on differential expression of 1-aminocyclopropane-1-carboxylic acid oxidase genes in table grape cv. Thompson Seedless. (2013) Postharvest Biology and Technology, 76. 163-169. ISSN 0925-5214

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Official URL: http://dx.doi.org/10.1016/j.postharvbio.2012.10.006

Abstract

As a consequence of the non-climacteric status of grapes (Vitis vinifera), ethylene biosynthesis and signal transduction have scarcely been studied in this fruit. In spite this drawback, the available information suggests a role for ethylene in ripening grape berries. In this work, we report the identification of three homologous genes that encode 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), a key component of ethylene biosynthesis. A comparison of protein sequences revealed that all three VvACOs harbor a 2OG-Fe(II) oxygenase domain, which is typical of the ACO gene family; however, VvACO1 showed a higher amino acid sequence homology with VvACO2 than with VvACO3. The expression pattern of VvACOs and the effect of exogenous ethylene on their transcript accumulation were evaluated during table grape berry development in the “Thompson Seedless” cultivar. A peak in VvACO1 transcript accumulation levels was registered around veraison that was 4-fold higher than at harvest, and this peak was confirmed during a second season in grapes that were harvested from three different vineyards. An enhancement in ethylene production and VvACO genes transcript levels was observed in grapes sprayed with ethephon during berry development. However, VvACO1 transcripts reached the highest accumulation earlier than VvACO2 and VvACO3. Altogether, these data confirmed that ethylene may have a role in some aspects of the grape ripening process, and they also highlighted the potential use of some VvACO genes as molecular markers for identifying grape veraison stages in grapes.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com/science/article/pii/S0925521412002359
HAL Id:hal-00906318
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Instituto de Investigaciones Agropecuarias - INIA (CHILE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > Universidad Andrés Bello - UNAB (CHILE)
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Deposited On:25 Jun 2013 07:53

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