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Analysis of gene mutation in plant cell wall by dielectric relaxation

Roig, Frédéric and Dantras, Eric and Grima-Pettenati, Jacqueline and Lacabanne, Colette Analysis of gene mutation in plant cell wall by dielectric relaxation. (2012) Journal of Physics D: Applied Physics, 45 (29). 1-7. ISSN 0022-3727

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Official URL: http://dx.doi.org/10.1088/0022-3727/45/29/295402


Arabidopsis Thaliana is a plant composed mainly of cellulose and lignin. Geneticists need techniques able to make differences at the molecular level between modified plants (DML6, CAD C/D) and non-modified ones. Thermo-stimulated current (TSC) analysis is a promising route to identify gene mutations. For the non-modified plant, at low temperatures, TSC thermograms highlight three dielectric relaxation modes. From −150 to −110 ◦C, γCellulose is attributed to CH2OH and –OH groups of cellulose. Between −110 and −80 ◦C, βLignin is detected. From −80 to −40 ◦C, βCellulose is characteristic of the molecular mobility of glycosidic linkages. For the CAD C/D modified plants, only γCellulose and βLignin are observed; due to analogous enthalpy values, those modes have the same molecular origin as in the non-modified plant. So, the βLignin mode is associated with the molecular mobility of the lignin-OH groups. The CAD C/D gene mutation changes the chemical structure of lignin, which promotes hydrogen bonds in the network and inhibits molecular mobility of glucosidic rings. It is also interesting to note that the DML6 gene mutation induces a higher cooperativity of this βCellulose relaxation than in wild vegetal composites. In fact, this mutation promotes molecular mobility of glycosidic rings thanks to β1–4 glycosidic linkages.

Item Type:Article
Additional Information:Thanks to Institute of Physics editor. The definitive version is available at http://iopscience.iop.org The original PDF of the article can be found at Journal of Physics D: Applied Physics website : http://dx.doi.org/10.1088/0022-3727/45/29/295402
HAL Id:hal-00880037
Audience (journal):International peer-reviewed journal
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Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
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Deposited On:03 Sep 2013 13:05

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