Labour, Marie-Noëlle and Banc, Amélie and Tourrette, Audrey and Cunin, Frédérique and Verdier, Jean-Michel and Devoisselle, Jean-Marie and Marcilhac, Anne and Belamie, Emmanuel Thick collagen-based 3D matrices including growth factors to induce neurite outgrowth. (2012) Acta Biomaterialia, 8 (9). 3302-3312. ISSN 1742-7061
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(Document in English)
PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 11MB |
Official URL: http://dx.doi.org/10.1016/j.actbio.2012.05.015
Abstract
Designing synthetic microenvironments for cellular investigations is a very active area of research at the crossroads of cell biology and materials science. The present work describes the design and functionalization of a three-dimensional (3D) culture support dedicated to the study of neurite outgrowth from neural cells. It is based on a dense self-assembled collagen matrix stabilized by 100-nm wide interconnected native fibrils without chemical crosslinking. The matrices were made suitable for cell manipulation and direct observation in confocal microscopy by anchoring them to traditional glass supports with a calibrated thickness of ∼50 μm. The matrix composition can be readily adapted to specific neural cell types, notably by incorporating appropriate neurotrophic growth factors. Both PC-12 and SH-SY5Y lines respond to growth factors (nerve growth factor and brain-derived neurotrophic factor, respectively) impregnated and slowly released from the support. Significant neurite outgrowth is reported for a large proportion of cells, up to 66% for PC12 and 49% for SH-SY5Y. It is also shown that both growth factors can be chemically conjugated (EDC/NHS) throughout the matrix and yield similar proportions of cells with longer neurites (61% and 52%, respectively). Finally, neurite outgrowth was observed over several tens of microns within the 3D matrix, with both diffusing and immobilized growth factors.
Item Type: | Article |
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Additional Information: | Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF can be found at Acta Biomaterialia website: http://www.sciencedirect.com/science/article/pii/S174270611200219X |
Audience (journal): | International peer-reviewed journal |
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Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Other partners > Ecole Nationale Supérieure de Chimie de Montpellier - ENSCM (FRANCE) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM (FRANCE) Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE) Other partners > Université de Montpellier 2 (FRANCE) Other partners > Ecole Pratique des Hautes Etudes - EPHE (FRANCE) |
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Deposited On: | 18 Sep 2013 10:28 |
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