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Phenotypes Associated with Down-Regulation of Sl-IAA27 Support Functional Diversity Among Aux/IAA Family Members in Tomato

Bassa, Carole and Mila, Isabelle and Bouzayen, Mondher and Delalande, Corinne Phenotypes Associated with Down-Regulation of Sl-IAA27 Support Functional Diversity Among Aux/IAA Family Members in Tomato. (2012) Plant and Cell Physiology, 53 (9). 1583-1595. ISSN 0032-0781

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Official URL: http://dx.doi.org/10.1093/pcp/pcs101

Abstract

The phytohormone auxin is known to regulate several aspects of plant development, and Aux/IAA transcription factors play a pivotal role in auxin signaling. To extend our understanding of the multiple functions of Aux/IAAs further, the present study describes the functional characterization of Sl-IAA27, a member of the tomato Aux/IAA gene family. Sl-IAA27 displays a distinct behavior compared with most Aux/IAA genes regarding the regulation of its expression by auxin, and the Sl-IAA27-encoded protein harbors a unique motif of unknown function also present in Sl-IAA9 and remarkably conserved in monocot and dicot species. Tomato transgenic plants underexpressing the Sl-IAA27 gene revealed multiple phenotypes related to vegetative and reproductive growth. Silencing of Sl-IAA27 results in higher auxin sensitivity, altered root development and reduced Chl content in leaves. Both ovule and pollen display a dramatic loss of fertility in Sl-IAA27 down-regulated lines,and the internal anatomy of the flower and the fruit are modified, with an enlarged placenta in smaller fruits. In line with the reduced Chl content in Sl-IAA27 RNA interference(RNAi) leaves, genes involved in Chl synthesis display lower expression at the level of transcript accumulation. Even though Sl-IAA27 is closely related to Sl-IAA9 in terms of sequence homology and the encoded proteins share common structural features, the data indicate that the two genes regulate tomato fruit initiation and development in a distinct manner.

Item Type:Article
Additional Information:Thanks to Oxford University Press editor. The definitive version is available at http://pcp.oxfordjournals.org/content/53/9/1583
Audience (journal):International peer-reviewed journal
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Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - INPT (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
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Deposited By: Brigitte LAFFORGUE
Deposited On:11 Dec 2012 09:01

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