Salameh, Dominique and Brandam, Cédric
and Rizk, Toufic and Lteif, Roger and Strehaiano, Pierre
Application of a reversed-phase HPLC method for quantitative P-Coumaric acid analysis in wine.
(2010)
Journal international des sciences de la vigne et du vin, 44 (2). 117-125. ISSN 1151-0285
|
(Document in English)
PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 304kB |
Abstract
Aims: This paper presents a rapid chromatographic method to monitor the concentration of p-coumaric acid in wine and in bioconversion studies. Methods and results: RP-HPLC method was validated in synthetic wine medium and in natural red wine. Mobile phase composition was water 77%, acetonitrile 23%. Formic acid was added to control pH at 3.5. The flow was 0.7 mL/min and the temperature 30 °C. The detection was done using UV at 305 nm. The linearity range was validated between 0.5 and 15 mg/L. The resolution was respectively 5.35 and 2.99. The detection and quantification limits were 0.01 mg/L and 0.04 mg/L. This method was used to study p-coumaric acid bioconversion into 4-ethylphenol and 4-vinylphenol, and to study this acid adsorption in enological conditions. Conclusions: This paper presented a simple HPLC method to monitor the concentration of p-coumaric acid in synthetic media and natural wine. It was used to study the p-coumaric acid bioconversion rates and mechanism. Significance and impact of the study: This method is useful to monitor p-coumaric acid concentration, which helps to predict amounts of 4-ethylphenol or 4-vinylphenol that can be produced in wine. This method can be helpful to control undesirable phenolic flavors potential in wine.
Item Type: | Article |
---|---|
Additional Information: | Thanks to Vigne et Vin Publications Internationales editor. The definitive version is available at http://www.jisvv.com The original PDF of the article can be found at : http://www.jisvv.com/moteur/moteur_avance.php# |
HAL Id: | hal-03474216 |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE) Other partners > Université Saint-Joseph de Beyrouth - USJ (LEBANON) |
Laboratory name: | Laboratoire de Génie Chimique - LGC (Toulouse, France) - Bioprocédés et systèmes microbiens (BioSyM) |
Statistics: | download |
Deposited On: | 04 Mar 2013 16:01 |
Repository Staff Only: item control page