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Protocol for the assessment of viral retention capability of membranes

Pierre, Gwenaelle and Furiga, Aurélie and Bergé, Mathieu and Roques, Christine and Aimar, Pierre and Causserand, Christel Protocol for the assessment of viral retention capability of membranes. (2011) Journal of Membrane Science, 381 (1-2). 41-49. ISSN 0376-7388

(Document in English)

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Official URL: http://dx.doi.org/10.1016/J.MEMSCI.2011.07.017


A series of experiments has been carried out to determine the Log removal value (LRV) of MS2 bacteriophages suspended in various buffers (osmosed water, tap water, aqueous solutions of NaCl and phosphate buffer solution) during filtration through hollow fiber membranes made of cellulose acetate. Viral concentrations in permeate and retentate were determined using two different methods, namely plaque forming unit (PFU) counting, which reveals only infectious particles and quantitative RT-PCR which detects the total (infectious + inactivated) number of viral genomes regardless of their infectivity.From this experimental study, we propose guidelines for preparing the challenging solutions and measuring their concentration which ensure a reliable assessment of the membrane performance.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at :http://www.sciencedirect.com/science/article/pii/S0376738811005163
HAL Id:hal-03539318
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Laboratory name:
Laboratoire de Génie Chimique - LGC (Toulouse, France) - Bioprocédés et systèmes microbiens (BioSyM)
Deposited On:28 Jun 2012 09:43

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