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Thidiazuron-induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars

Arshad, Muhammad and Silvestre, Jérôme and Merlina, Georges and Dumat, Camille and Pinelli, Eric and Kallerhoff, Jean Thidiazuron-induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars. (2012) Plant Cell, Tissue and Organ Culture, 108 (2). 315-322. ISSN 0167-6857

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Official URL: http://dx.doi.org/10.1007/s11240-011-0045-1


Shoot organogenesis from mature leaf tissues of two scented Pelargonium capitatum cultivars, ‘Attar of Roses’ and ‘Atomic Snowflake’, grown in the greenhouse, were optimized in the presence of thidiazuron (TDZ). The protocol involved preculture of leaf sections on basal Murashige and Skoog (MS) medium supplemented with 10 lM TDZ, 4.4 lM of 6-benzyladenine (BA) and 5.4 lM a-naphtaleneacetic acid (NAA) for a period of 2 weeks and followed by subculture of explants to a fresh medium containing 4.4 lM BA and 5.4 lM NAA. Frequency of regeneration reached approximately 93% for both cultivars, with the induction of more than 100 shoots per explant. Regenerated plantlets were rooted on half-strength MS medium supplemented with 4.4 mM sucrose and 8.6 lM of Indole-3-acetic acid (IAA). All regenerated shoots from both cultivars developed roots when transferred to organic soil mix, acclimatized, and successfully transferred to greenhouse conditions. When regenerated shoots were transferred to hydroponic conditions, frequency of survival was 76.2 and 61.9% for ‘Attar of Roses’ and ‘Atomic Snowflake’, respectively.

Item Type:Article
Additional Information:Thanks to Springer editor. The definitive version is available at http://www.springerlink.com/content/pl3t422186375210/?MUD=MP
HAL Id:hal-00716999
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Laboratory name:
Deposited On:11 Jul 2012 15:43

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