Aimar, Pierre and Meireles, Martine
Calibration of ultrafiltration membranes against size exclusion chromatography columns.
(2010)
Journal of Membrane Science, 346 (2). 233-239. ISSN 0376-7388
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(Document in English)
PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 352kB |
Official URL: http://dx.doi.org/10.1016/J.MEMSCI.2009.09.018
Abstract
Using the extension of the concept of universal calibration parameter, yielding a relation between the hydrodynamic volume of molecules and the elution volume in sizeexclusionchromatography (SEC), to retention coefficients in ultrafiltration (UF), we propose a direct calibration of UF membranes against chromatography columns. Plotting the retention coefficient by one given UF membrane of a series of probe molecules versus their elution volume in SEC chromatography provides a calibration curve for this membrane. For a wide range of retentions, such calibration can be directly used to predict the retention of any molecule: one only needs to measure its exclusion volume by the SEC column, and read the retention by the calibrated membrane on the calibration curves. The method has been tested with dextran and PEG for the calibration, and milk proteins as test molecules, for three different membranes. The predicted values of the retention are in rather good agreement with those experimentally measured in a UF cell.
Item Type: | Article |
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Additional Information: | Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at : http://www.sciencedirect.com/science/article/pii/S0376738809006747 |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE) |
Laboratory name: | Laboratoire de Génie Chimique - LGC (Toulouse, France) - Génie des Interfaces & Milieux Divisés (GIMD) |
Statistics: | download |
Deposited On: | 29 May 2012 07:32 |
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