Strub, Caroline and Pocaznoi, Diana
and Lebrihi, Ahmed
and Fournier, Richard and Mathieu, Florence
Influence of barley malting operating parameters on T-2 and HT-2 toxinogenesis of Fusarium langsethiae, a
worrying contaminant of malting barley in Europe.
(2010)
Food Additives and Contaminants: Part A: Chemistry,Analysis, Control, Exposure & Risk Assessment, 27 (9). 1247-1252. ISSN 1944-0049
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(Document in English)
PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 290kB |
Official URL: http://dx.doi.org/10.1080/19440049.2010.487498
Abstract
The fungus Fusarium langsethiae, exclusively described in Europe at present, seems to have taken the place of other Fusarium species in barley fields over the last 5 years. It has proved to be a highly toxic type-A trichothecene producer (T-2 and HT-2 toxins). The aim of this work was to study the ecotoxinogenesis of this fungus the better to identify and manage the health risk it may pose during the beer manufacturing process. The influence of temperature and water activity on its growth rate and production of toxins are particularly assessed from a macroscopic point of view. Different cultures were grown on sterilized rehydrated barley with a water activity between 0.630 and 0.997 and a temperature ranging from 5 to 35°C. Biomass specific to F. langsethiae and T-2 and HT-2 toxins were quantified by real-time polymerase chain reaction and liquid chromatography-mass spectrometry, respectively. It appears that the optimal temperature and water activity for F. langsethiae toxinogenesis are 28°C and 0.997. This fungus was able to produce 2.22 g kg−1 of these toxins in 16 days on barley in optimal production conditions. The malting process seems to be a critical step because, in its temperature range, specific production was six times higher than under optimal temperatures for fungus growth. In the short-term, this work will help redefine the process conditions for malting. In the medium-term, the results will contribute to the development of a molecular tool to diagnose the presence of this contaminant and the detection of the toxins in barley, from fields to the end product.
Item Type: | Article |
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Additional Information: | Thanks to Taylor & Francis. The definitive version is available at http://www.tandfonline.com The original PDF of the article can be found at : http://www.tandfonline.com/doi/abs/10.1080/19440049.2010.487498?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Other partners > Institut Français des Boissons, de la Brasserie et de la Malterie - IFBM (FRANCE) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE) |
Laboratory name: | Laboratoire de Génie Chimique - LGC (Toulouse, France) - Bioprocédés et systèmes microbiens (BioSyM) |
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Deposited On: | 25 May 2012 12:34 |
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