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Sessile Legionella pneumophila is able to grow on surfaces and generate structured monospecies biofilms

Pécastaings, Sophie and Bergé, Mathieu and Dubourg, Karine and Roques, Christine Sessile Legionella pneumophila is able to grow on surfaces and generate structured monospecies biofilms. (2010) Biofouling, 26 (7). 809-819. ISSN 0892-7014

(Document in English)

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Official URL: http://dx.doi.org/10.1080/08927014.2010.520159


Currently, models for studying Legionella pneumophila biofilm formation rely on multi-species biofilms with low reproducibility or on growth in rich medium, where planktonic growth is unavoidable. The present study describes a new medium adapted to the growth of L. pneumophila monospecies biofilms in vitro. A microplate model was used to test several media. After incubation for 6 days in a specific biofilm broth not supporting planktonic growth, biofilms consisted of 5.36 ± 0.40 log (cfu cm−2) or 5.34 ± 0.33 log (gu cm−2). The adhered population remained stable for up to 3 weeks after initial inoculation. In situ confocal microscope observations revealed a typical biofilm structure, comprising cell clusters ranging up to 300 μm in height. This model is adapted to growing monospecies L. pneumophila biofilms that are structurally different from biofilms formed in a rich medium. High reproducibility and the absence of other microbial species make this model useful for studying genes involved in biofilm formation.

Item Type:Article
Additional Information:Thanks to Taylor and Francis editor. The definitive version is available at http://www.tandfonline.com/. The original PDF of the article can be found at : http://www.tandfonline.com/doi/abs/10.1080/08927014.2010.520159
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université de Bordeaux 2 - Victor Segalen (FRANCE)
Laboratory name:
Deposited On:22 May 2012 09:40

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