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Hydrated elastin: Dynamics of water and protein followed by dielectric spectroscopies

Samouillan, Valérie and Tintar, Doris and Lacabanne, Colette Hydrated elastin: Dynamics of water and protein followed by dielectric spectroscopies. (2011) Chimical Physics, 385 (1-3). 19-26. ISSN 0301-0104

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Official URL: http://dx.doi.org/10.1016/j.chemphys.2011.04.016


The dielectric properties of elastin were investigated at different levels of hydration and specifically at the limit of freezable water apparition. The quantification of freezable water was performed by Differential Scanning Calorimetry (DSC). Two dielectric techniques were used to explore the dipolar relaxations of hydrated elastin: dynamic dielectric spectroscopy (DDS), performed isothermally with the frequency varying from 10(-2) to 3.10(-6) Hz, and the technique of thermally stimulated depolarization currents (TSDC), an isochronal spectrometry running at variable temperature, analogous to a low frequency spectroscopy {10(-3)– 10(-2) Hz}. A complex relaxation map was evidenced by the two techniques. Assignments for the different processes can be proposed by the combination of DDS and TSDC experiments and the determination of the activation parameters of the relaxation times. As already observed for globular proteins, the concept of "solvent-slaved" protein motions is checked for the fibrillar hydrated elastin.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The orinal version is available at http://www.sciencedirect.com The original PDF of the article can be found at Chimical Physics website: http://www.sciencedirect.com/science/article/pii/S0301010411001352
HAL Id:hal-03541395
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
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Deposited On:15 May 2012 17:02

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