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Safety and immunogenicity of myxoma virus as a new viral vector for small ruminants

Pignolet, Béatrice and Boullier, Séverine and Gelfi, Jacqueline and Bozzetti, Marjorie and Russo, Pierre and Foulon, Eliane and Meyer, Gilles and Delverdier, Maxence and Foucras, Gilles and Bertagnoli, Stéphane Safety and immunogenicity of myxoma virus as a new viral vector for small ruminants. (2008) Journal of General Virology, 89. 1371-1379. ISSN 0022-1317

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Official URL: http://dx.doi.org/10.1099/vir.0.83595-0


Myxoma virus (MYXV), a leporide-specific poxvirus, represents an attractive candidate for the generation of safe and non-replicative vaccine vectors for other species. With the aim of developing new recombinant vaccines for ruminants, we evaluated the safety and the immunogenicity of recombinant MYXV in sheep. In vitro studies indicated that ovine primary fibroblasts were not permissive for MYXV and that infection of ovine peripheral blood mononuclear cells occurred at a low rate. Although non-specific activation significantly improved the susceptibility of lymphocytes, MYXV infection remained abortive. Histological and immunohistochemical examination at the inoculation sites revealed the development of an inflammatory process and allowed the detection of sparse infected cells in the dermis. In addition, inoculated sheep developed an antibody response directed against MYXV and the product of the transgene. Overall, these results provide the first line of evidence on the potential of MYXV as a viral vector for ruminants.

Item Type:Article
Additional Information:Thanks to Society for General Microbiology editor. The original PDF of the article can be found at : http://vir.sgmjournals.org/cgi/content/abstract/89/6/1371
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Agence Française de Sécurité Sanitaire des Aliments - AFSSA (FRANCE)
Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
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Deposited On:18 May 2011 09:36

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