Leberre, Véronique and Baranowski, Eric and Deplanche, Martine and Trouilh, Lidwine and François, Jean Marie Detection of minority variants within bovine respiratory syncytial virus populations using oligonucleotide-based microarrays. (2008) Journal of Virological Methods, 148. 271-276. ISSN 0166-0934
|
(Document in English)
PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 883kB |
Official URL: http://dx.doi.org/10.1016/j.jviromet.2007.10.026
Abstract
Microarray technology, originally developed for highly parallel examination of gene expression is regarded as a potential tool in prognosis and diagnosis. With respect to a discrimination analysis, difference as small as one nucleotide base can be distinguished using oligonucleotide-basedmicroarrays. However, this degree of specificity is dependent on several parameters, including the size of the oligoprobes and the sequence context of the probes (e.g. local melting temperature), hybridization conditions and to some extent the chemistry of the glass slides onto which the probes are deposited. Using bovine respiratory syncytial virus (BRSV) as a model study, an oligonucleotide-based microarray approach was developed to measure the relative abundance of a particular single nucleotide variant within mixed BRSV populations. Using this technology, we show that it is possible to discriminate at a rate of 1%, minority variants in a BRSV population.
Item Type: | Article |
---|---|
Additional Information: | Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Journal of Virological Methods : http://bit.ly/lITn6Q |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT (FRANCE) French research institutions > Institut National de la Recherche en Informatique et en Automatique - INRIA (FRANCE) Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE) Other partners > Platform Toulouse Genopole (FRANCE) |
Laboratory name: | |
Statistics: | download |
Deposited On: | 23 May 2011 08:31 |
Repository Staff Only: item control page