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Adsorption and release of BMP-2 on nanocrystalline apatite-coated and uncoated hydroxyapatite/b-tricalcium phosphate porous ceramics

Autefage, Hélène and Briand-Mésange, Fabienne and Cazalbou, Sophie and Drouet, Christophe and Fourmy, Daniel and Gonçalvès, Stéphane and Salles, Jean-Pierre and Combes, Christèle and Swider, Pascal and Rey, Christian Adsorption and release of BMP-2 on nanocrystalline apatite-coated and uncoated hydroxyapatite/b-tricalcium phosphate porous ceramics. (2009) Journal of Biomedical Materials Research Part B: Applied Biomaterials, 91B (2). 706-715. ISSN 1552-4973

(Document in English)

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Official URL: http://dx.doi.org/10.1002/jbm.b.31447


The association of bone morphogenetic proteins (BMPs) with calcium phosphate bioceramics is known to confer them osteoinductive properties. The aim of this study was to evaluate the surface properties, especially regarding recombinant human BMP-2 (rhBMP-2) adsorption and release, of commercial sintered biphasic calcium phosphate ceramics after coating with biomimetic nanocrystalline apatite. The raw and coated ceramics exhibited similar macroporous structures but different nanometer-sized pores contents. Both types of ceramics showed Langmuir-type adsorption isotherms of rhBMP-2. The coating noticeably increased the rate of adsorption and the total amount of growth factor taken up, but the maximum coverage per surface area unit as well as the affinity constant appeared lower for coated ceramics compared with raw ceramic surfaces. The limited advantage gained by coating the ceramics can be assigned to a lower accessibility of the surface adsorption sites compared with the raw ceramics. The quantity of rhBMP-2 spontaneously released in cell culture medium during the first weeks was lower for coated samples than for uncoated ceramics and represented a minor fraction of the total adsorbed amount. In conclusion, the nanocrystalline apatite coating was found to favor the adsorption of rhBMP-2 while providing a mean to fine tune the release of the growth factor.

Item Type:Article
Additional Information:Thanks to John Wiley & Sons editor. The definitive version is available at http://eu.wiley.com/ The original PDF of the article can be found at Journal of Biomedical Materials Research Part B: Applied Biomaterials website : http://www3.interscience.wiley.com/journal/122499095/abstract
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM (FRANCE)
Other partners > TEKNIMED (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Centre Hospitalier Universitaire de Toulouse - CHU Toulouse (FRANCE)
Laboratory name:
Deposited On:23 Feb 2011 13:55

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