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Prion protein in the cerebrospinal fluid of healthy and naturally scrapie-affected sheep

Picard-Hagen, Nicole and Gayrard, Véronique and Viguié, Catherine and Moudjou, Mohammed and Imbs, Chantal and Toutain, Pierre-Louis Prion protein in the cerebrospinal fluid of healthy and naturally scrapie-affected sheep. (2006) Journal of General Virology, 8. 3723-3727. ISSN 0022-1317

(Document in English)

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Official URL: http://dx.doi.org/10.1099/vir.0.81859-0


The aim of this study was to characterize the cerebrospinal fluid (CSF) prion protein (PrP) of healthy and naturally scrapie-affected sheep. The soluble form of CSF PrPC immunoblotted with an anti-octarepeat and an anti-C terminus mAb showed two isoforms of approximately 33 and 26 kDa, corresponding to the biglycosylated and unglycosylated isoforms of brain PrPC. Neither the mean concentration nor the electrophoretic profile of CSF PrP differed between healthy and scrapieaffected sheep, whereas a slightly increased resistance of CSF PrP to mild proteolysis by proteinase K was evident in the CSF of scrapie-affected sheep. No difference in susceptibility to proteolysis was observed between the two ARR and VRQ genetic variants of the purified prokaryote recombinant PrP. It was concluded that the physicochemical properties of PrPC in the CSF could be altered during scrapie and that these changes might reflect the physiopathological process of prion disease.

Item Type:Article
Additional Information:Thanks to the Society for General Microbiology. The definitive version is available at http://vir.sgmjournals.org/. The original PDF of the article can be found at Journal of General Virology website: http://vir.sgmjournals.org/cgi/reprint/87/12/3723.pdf
Audience (journal):International peer-reviewed journal
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Institution:Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT (FRANCE)
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Deposited On:20 May 2010 09:11

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