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Fabrication of hydroxyapatite sponges by dextran sulphate/amino acid templating

Gonzalez-McQuire, Rosanna and Green, David and Walsh, Dominic and Hall, Simon and Chane-Ching, Jean-Yves and Oreffo, Richard O.C. and Mann, Stephen Fabrication of hydroxyapatite sponges by dextran sulphate/amino acid templating. (2005) Biomaterials, 26 (33). 6652-6656. ISSN 0142-9612

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Official URL: http://dx.doi.org/10.1016/j.biomaterials.2005.04.037


We report a new template-directed method for the fabrication of hydroxyapatite (HAp) sponges by using amino-acid-coated HAp nanoparticles dispersed within a viscous polysaccharide (dextran sulfate) matrix, and describe the use of these materials for the viability and proliferation of human bone marrow stromal cells. The nanoparticles were prepared in the presence of excess amounts of aspartic acid, alanine or arginine, and subsequently organised into macroporous frameworks with typical pore sizes of 100–200 mm during thermal degradation of the dextran matrix. The sponge macrostructure was influenced by changes in the heating rate and sintering time, as well as the use of different amino acids or variations in dextran functional groups. Biocompatibility testing showed retention of cell viability, production of extracellular matrix and alkaline phosphatase expression, suggesting that it should be possible to exploit this novel fabrication method for potential applications in cartilage or soft tissue engineering.

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Biomaterials website : http://www.sciencedirect.com/science/journal/01429612
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > University of Bristol (UNITED KINGDOM)
Other partners > Rhodia (FRANCE)
Other partners > University of Southampton (UNITED KINGDOM)
Laboratory name:
Deposited On:09 Jul 2010 14:11

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