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New direct contact approach to evaluate soil genotoxicity using the Vicia faba micronucleus test.

Marcato, Claire-Emmanuelle and Guiresse, Agnès Maritchù and Cecchi, Marie and Cotelle, Sylvie and Pinelli, Eric New direct contact approach to evaluate soil genotoxicity using the Vicia faba micronucleus test. (2009) Chemosphere, 77 (3). 345-350. ISSN 0045-6535

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Official URL: http://dx.doi.org/10.1016/j.chemosphere.2009.07.016


A method to assess micronucleus (MN) induction in Vicia faba roots by direct contact exposure to a solid matrix was developed. The procedure comprised a 5-d germination period, as in the well-known method using aqueous extracts. However, the seeds were here sown directly into the test soil whereas a culture period is necessary before exposing seedlings to a liquid medium. One soil under forest and two contaminated soils from areas affected by industrial installations and a coke works were used. Three durations of direct exposure were tested: 2, 5 and 7 d. The optimal duration was evaluated at 2 d to observe maximal MN induction without observing toxicity symptoms. The methodology using aqueous extracts was applied to the same three soils: MN frequency was higher than in the direct contact assay but the ratios of MN frequencies from tested soils in comparison to the negative control were lower. However, for each soil, both the direct contact method and the aqueous extract exposure led to the same risk assessment diagnosis. The evaluation of a concentration range of a polycyclic aromatic hydrocarbons (PAH)-contaminated soil showed a dose-dependent MN frequency when the seeds were allowed to germinate before sowing in the soil: the soil genotoxicity was the highest at intermediate doses. The direct contact method was found to be rapid, sensitive and well suited to the evaluation of soil quality

Item Type:Article
Additional Information:Thanks to Elsevier editor. The definitive version is available at http://www.sciencedirect.com The original PDF of the article can be found at Chemosphere website :http://www.elsevier.com/wps/find/journaldescription.cws_home/362/description#description
HAL Id:hal-03574185
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université Paul-Verlaine - Metz (FRANCE)
Laboratory name:
Deposited On:17 Dec 2010 16:11

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