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A new cell primo-culture method for freshwater benthic diatom communities

Debenest, Timothée and Silvestre, Jérôme and Coste, Michel and Delmas, François and Pinelli, Eric A new cell primo-culture method for freshwater benthic diatom communities. (2009) Journal of Applied Psychology, 21 (1). 65-73. ISSN 0921-8971

(Document in English)

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Official URL: http://dx.doi.org/10.1007/s10811-008-9325-5


A new cell primo-culture method was developed for the benthic diatom community isolated from biofilm sampled in rivers. The approach comprised three steps: (1) scraping biofilm from river pebbles, (2) diatom isolation from biofilm, and (3) diatom community culture. With a view to designing a method able to stimulate the growth of diatoms, to limit the development of other microorganisms, and to maintain in culture a community similar to the original natural one, different factors were tested in step 3: cell culture medium (Chu No 10 vs Freshwater “WC” medium modified), cell culture vessel, and time of culture. The results showed that using Chu No 10 medium in an Erlenmeyer flask for cell culture was the optimal method, producing enough biomass for ecotoxicological tests as well as minimising development of other microorganisms. After 96 h of culture, communities differed from the original communities sampled in the two rivers studied. Species tolerant of eutrophic or saprobic conditions were favoured during culture. This method of diatom community culture affords the opportunity to assess, in vitro, the effects of different chemicals or effluents (water samples andindustrial effluents) on diatom communities, as well as on diatom cells, from a wide range of perspectives.

Item Type:Article
Additional Information:Thanks to Springer editor. The definitive version is available at http://www.springerlink.com/ The original PDF of the article can be found at Journal of Applied Psychology website : http://www.springerlink.com/content/0921-8971/
Audience (journal):International peer-reviewed journal
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Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
French research institutions > Institut national de recherche en sciences et technologies pour l’environnement et l’agriculture - CEMAGREF (FRANCE)
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Deposited On:04 Jul 2011 14:33

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