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Lipopeptide overproduction by cell immobilization on iron-enriched light polymer particles

Gancel, Frédérique and Montastruc, Ludovic and Liu, Tao and Zhao, Ling and Nikov, Iordan Lipopeptide overproduction by cell immobilization on iron-enriched light polymer particles. (2009) Process Biochemistry, 44 (9). 975-978. ISSN 1359-5113

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Official URL: http://dx.doi.org/10.1016/j.procbio.2009.04.023


The study concerns surfactin and/or fengycin batch production by immobilized cells of Bacillus subtilis ATCC 21332. Light carriers designed for a three phase inverse fluidized bed biofilm reactor (TPFIBR) were used. With respect to the biofilm reactor development, a new support based on iron grafting onto polypropylene foams has been proposed. A suspension solid-state grafting process was applied to graft ferric acetylacetonate onto polypropylene (PP) foams with a density of 0.3–0.7 g/cm3. The iron contents grafted onto PP increased with the reaction time and then it tended to level off. The iron contents at 7.5 and 10 h are 0.74 and 0.75 wt%, respectively. It was specified that the equilibrium was reached at 7.5 h. Influence of particles on lipopeptide production was analyzed in two kinds of experiments: preliminary colonization step of particles, followed by production step in modified culture medium (named in this work colonization step) or direct addition of pellets in culturemedium (named production step). All PP+ iron pellets promoted biomass enhancement. The production yield was modified for all types of PP supports, containing respectively 0, 0.35 and 0.75% of iron. The immobilized cultures produced 2.09–4.3 times more biosurfactants than planktonic cells. In production experiments addition of carriers seemed tomodify the ratio between surfactin and fengycin with an enhancement of the fengycin production. The highest concentration of fengycin was obtained with addition of pellets containing 0.35% of iron.

Item Type:Article
Additional Information:Thanks to Elsevier editor. This article is available at http://www.sciencedirect.com. The PDF of the article can be found at Process Biochemistry website : http://www.sciencedirect.com/science/journal/13595113
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Polytech'Lille (FRANCE)
Other partners > Université Lille 1, Sciences et Technologies - Lille 1 (FRANCE)
Other partners > East China University of Science and Technology - ECUST (CHINA)
Laboratory name:
Deposited On:14 Sep 2009 09:50

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