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Beef meat and blood sausage promote azoxymethane-induced mucin-depleted foci and aberrant crypt foci in rat colons

Pierre, Fabrice and Freeman, Amanda and Taché, Sylviane and Van der Meer, Roleof and Corpet, Denis E. Beef meat and blood sausage promote azoxymethane-induced mucin-depleted foci and aberrant crypt foci in rat colons. (2004) The Journal of Nutrition, 134. 2711-2716. ISSN 0022-3166

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Official URL: http://jn.nutrition.org/cgi/reprint/134/10/2711?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&author1=corpet&fulltext=Beef+meat+and+blood+sausage+promote+azoxymethane-induced+mucin-depleted+foci+and&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCIT


Red meat intake is associated with colon cancer risk. Puzzlingly, meat does not promote carcinogenesis in rat studies. However, we demonstrated previously that dietary heme promotes aberrant crypt foci (ACF) formation in rats given a low-calcium diet. Here, we tested the hypothesis that heme-rich meats promote colon carcinogenesis in rats treated with azoxymethane and fed low-calcium diets (0.8 g/kg). Three meat-based diets were formulated to contain varying concentrations of heme by the addition of raw chicken (low heme), beef (medium heme), or black pudding (blood sausage; high heme). The no-heme control diet was supplemented with ferric citrate and the heme control diet with hemoglobin to match iron and heme concentrations in the beef diet, respectively. After 100 d, colons were scored for ACF and mucin-depleted foci (MDF). Fecal water was assayed for lipoperoxides and cytotoxicity. Only diets with heme promoted the formation of MDF, but all meat diets promoted ACF formation. The number of MDF/colon was 0.55 +/- 0.68 in controls, but 1.2 +/- 0.6 (P = 0.13), 1.9 +/- 1.4 (P < 0.01), and 3.0 +/- 1.2 (P < 0.001) in chicken-, beef-, and black pudding-fed rats. MDF promotion by the high-heme black pudding diet was greater than that by the medium-heme beef diet. The number of ACF/colon was 72 +/- 16 in controls, but 91 +/- 18, 100 +/- 13, and 103 +/- 14 in chicken-, beef-, and black pudding-fed rats (all P < 0.001). ACF and MDF did not differ between rats fed the beef diet and those fed the heme control diet. MDF promotion was correlated with high fecal water lipoperoxides and cytotoxicity (r = 0.65, P < 0.01). This is the first study to show the promotion of experimental carcinogenesis by dietary meat and the association with heme intake.

Item Type:Article
Additional Information:The definitive version is available at http://jn.nutrition.org/ The original PDF of the article can be found at American society for nutrition website : http://tinyurl.com/lh69py
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > Wageningen Centre for Food Sciences - WCFS (NETHERLANDS)
Other partners > University of Melbourne (AUSTRALIA)
Université de Toulouse > Ecole Nationale Vétérinaire de Toulouse - ENVT (FRANCE)
Laboratory name:
Deposited On:16 Sep 2009 11:20

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