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Design d'un bioprocédé pour la valorisation des résidus d'agave pour la production de molécules plateforme

Manzanero Hoyos, Lauren alejandrina. Design d'un bioprocédé pour la valorisation des résidus d'agave pour la production de molécules plateforme. PhD, Génie des Procédés et de l'Environnement, Institut National Polytechnique de Toulouse, 2020, 133 p.

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Abstract

The concept of Biorefinery first appeared in the late 1980s as a plan to cope with climate change and the oil crisis in the world. However, several issues keep biorefineries from becoming competitive in the market. In this work, we focused on two issues resurfaced over the years to overcome them and be competitive in the market. These are: 1) The use of the lignocellulosic hydrolysates without subsequent C5 and C6 sugars separation stages and, 2) The production of multiple value-added molecules to minimize investing costs and be competitive against the molecules obtained from oil. The raw material we focus on is a model medium of agave bagasse. In fact, the production of alcoholic agave-based beverages increases each year, generating up to 40% of residues per liter produced. The strategy presented during this work would be the conception of a process for the production of two value-added metabolites, each coming from a type of sugar in the mixture. For each metabolite, one strain has been selected based on yield, productivity and its ability to be cultivated in compatible fermentation conditions with the other strain. With these criteria, strains of Candida guillermondii and Corynebacterium glutamicum were chosen. Three different propositions of fermentation processes were tested: mixed, sequential, and sequential with the removal of the first microorganism. C. glutamicum produced lactic acid at a yield of 1.3mol/mol after 48 h of culture. Proposed fermentation conditions for xylitol production by C guilliermondii in a media with a mix of sugars and lactic acid produced in the first stage of the sequential fermentation are: 0.1 vvm, initial pH adjusted at 7 without regulation. Under these conditions xylitol was produced at a yield of 0.63 mol/mol after 63 h of culture.

Item Type:PhD Thesis
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Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
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Research Director:
Brandam, Cédric and Joannis-Cassan, Claire
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Deposited On:22 Sep 2021 13:18

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