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Use of FT‐NIR and XPS techniques to distinguish cell hull fractions prepared by autolysis or HPH from Saccharomyces cerevisiae and Brettanomyces bruxellensis strains

Bakhos, Elena and Skaff, Wadih and Esvan, Jérôme and Monnier, Alexandre and Sieczkowski, Nathalie and Lteif, Roger and Brandam, Cédric and Salameh, Dominique Use of FT‐NIR and XPS techniques to distinguish cell hull fractions prepared by autolysis or HPH from Saccharomyces cerevisiae and Brettanomyces bruxellensis strains. (2021) International Journal of Food Science & Technology. 1-9. ISSN 0950-5423

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Official URL: https://doi.org/10.1111/ijfs.15318

Abstract

Yeast hulls can be used to adsorb undesirable compounds such as volatile phenols that may be present in wine. To understand this adsorption process, the properties of the cell walls and their chemical composition need to be better understood. A study was conducted using four different yeast fractions of either autolysed or high-pressure homogeniser (HPH)-crushed yeast biomasses of Saccharomyces cerevisiae and Brettanomyces bruxellensis. Near-infrared spectroscopy (FT-NIR) coupled with X-ray photoelectron spectroscopy (XPS) was used and analysed by principal component analysis (PCA). The FT-NIR spectral region of Saccharomyces and Brettanomyces statistically analysed by PCA showed a clear discrimination accounting for 76% of the variation in the data for PC1; moreover, yeast hulls prepared from the same strain and subjected to two different treatments were also separated. These methods classify yeast cell hulls (YCH) according to strain, composition and treatment applied. Our results indicate that yeast hulls obtained by autolysis are less rich in proteins than those resulting from HPH treatment due to the high pressure that releases more proteins and exposes them on the surface of the cell wall. The composition of YCH at the extreme surface is similar to that found deeper in the wall.

Item Type:Article
HAL Id:hal-03351817
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Lallemand SAS (FRANCE)
Other partners > Université Saint-Joseph de Beyrouth - USJ (LEBANON)
Laboratory name:
Funders:
Agence Nationale de la Recherche Technique - ANRT
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Deposited On:22 Sep 2021 13:52

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