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Nanoscale wetting of single viruses

Calò, Annalisa and Eleta-Lopez, Aitziber and Ondarçuhu, Thierry and Verdaguer, Albert and Bittner, Alexander M. Nanoscale wetting of single viruses. (2021) Molecules, 26 (17). 5184. ISSN 1420-3049

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Official URL: https://doi.org/10.3390/molecules26175184

Abstract

The epidemic spread of many viral infections is mediated by the environmental conditions and influenced by the ambient humidity. Single virus particles have been mainly visualized by atomic force microscopy (AFM) in liquid conditions, where the effect of the relative humidity on virus topography and surface cannot be systematically assessed. In this work, we employed multifrequency AFM, simultaneously with standard topography imaging, to study the nanoscale wetting of individual Tobacco Mosaic virions (TMV) from ambient relative humidity to water condensation (RH > 100%). We recorded amplitude and phase vs. distance curves (APD curves) on top of single virions at various RH and converted them into force vs. distance curves. The high sensitivity of multifrequency AFM to visualize condensed water and sub-micrometer droplets, filling gaps between individual TMV particles at RH > 100%, is demonstrated. Dynamic force spectroscopy allows detecting a thin water layer of thickness ⁓1 nm, adsorbed on the outer surface of single TMV particles at RH < 60%.

Item Type:Article
Additional Information:This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
HAL Id:hal-03345637
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > CIC nanoGUNE Consolider (SPAIN)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Consejo Superior de Investigaciones Científicas - CSIC (SPAIN)
Other partners > Institute for Bioengineering of Catalonia - IBEC (SPAIN)
Other partners > IKERBASQUE (SPAIN)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Universitat de Barcelona - UB (SPAIN)
Laboratory name:
Funders:
Diputación Foral de Guipuzcoa (SPAIN) - Basque government (SPAIN) - MCIU-MINECO (SPAIN)
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Deposited On:09 Sep 2021 13:47

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