OATAO - Open Archive Toulouse Archive Ouverte Open Access Week

Adaptation of neutrophilic Paracoccus denitrificans to denitrification at highly alkaline pH

Albina, Pierre and Durban, Nadège and Bertron, Alexandra and Schiettekatte, Maud and Albrecht, Achim and Robinet, Jean-Charles and Erable, Benjamin Adaptation of neutrophilic Paracoccus denitrificans to denitrification at highly alkaline pH. (2020) Environmental Science and Pollution Research, 27 (17). 22112-22119. ISSN 0944-1344

[img]
Preview
(Document in English)

PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
543kB

Official URL: https://doi.org/10.1007/s11356-020-08360-9

Abstract

Bacterial denitrification is widely documented at neutral pH in order to improve the removal of nitrate in wastewater treatment processes. However, certain industrial contexts generate alkaline waste and effluent containing nitrate that must be denitrified. To obtain more information on denitrification at alkaline pH, this study evaluated the possibility of adapting a neutrophilic denitrifying strain, Paracoccus denitrificans, to alkaline pH. Firstly, P. denitrificans’ denitrifying activity was evaluated without acclimation in batch bioreactors at pH 7.0, 8.0, 9.0 and 10.0. Then, two acclimation methods using successive batch bioreactors and a continuous bioreactor allowed P. denitrificans to be gradually exposed to alkaline pH: from 8.5 to 11.2 in 26 and 72 days respectively. Results showed that P. denitrificans could grow and catalyse nitrate reduction (i) at pH 9.0 without acclimation, (ii) at pH 10.5 in successive batch cultures with progressively increasing pH and (iii) at pH 10.8 in continuously fed culture with a hydraulic retention time (HRT) of 8 days. It was shown that denitrification affected the pH despite the presence of carbonate buffering of the P. denitrificans growth medium. With acetate as an electron donor, the pH of a carbonate buffered medium tends towards pH 10 during the process of denitrification.

Item Type:Article
HAL Id:hal-02617065
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Agence Nationale pour la Gestion des Déchets Radioactifs - ANDRA (FRANCE)
French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Institut National des Sciences Appliquées de Toulouse - INSA (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Laboratory name:
Funders:
Agence Nationale pour la Gestion des Déchets Radioactifs - ANDRA (FRANCE) - Université de Toulouse
Statistics:download
Deposited On:21 Jun 2021 10:39

Repository Staff Only: item control page