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Luminescent nanohybrids based on silica and silylated Ru(II)—Yb(III) heterobinuclear complex: new tools for biological media analysis

Sábio, Rafael Miguel and Santagneli, Silvia Helena and Gressier, Marie and Caiut, José Maurício Almeida and Pazin, Wallance Moreira and Leite, Ilaiali Souza and Inada, Natalia Mayumi and Rosa da Silva, Robson and Ribeiro, Sidney José Lima and Menu, Marie-Joëlle Luminescent nanohybrids based on silica and silylated Ru(II)—Yb(III) heterobinuclear complex: new tools for biological media analysis. (2019) Nanotechnology, 31 (8). 085709. ISSN 0957-4484

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Official URL: https://doi.org/10.1088/1361-6528/ab55c3

Abstract

Lanthanide (Ln) complexes emitting in the near-infrared (NIR) region have fostered great interest as upcoming optical tags owing to their high spatial and temporal resolution emission as well deeper light penetration in biological tissues for non-invasive monitoring. For use in live-cell imaging, lanthanide complexes with long-wavelength absorption and good brightness are especially critical. Light-harvesting ligands of Ln complexes are typically excited in the ultraviolet region, which in turn trigger simultaneously autofluorescence and long-exposition damage of living systems. The association of d-metalloligands rather than organic chromophores enables the excitation of NIR-emitting Ln complex occurs in the visible region. Taking advantage of the long-lived excited states and intense absorption band in the ultraviolet (UV) to NIR region of Ru(II), we successfully design a dual-emitting (in the visible and NIR region) d–f heterobinuclear complex based on Ru(II) metalloligand and Yb(III) complex. In addition, we developed luminescent nanohybrids by grafting of Ru(II)–Yb(III) heterobinuclear complexes containing silylated ligands on the surface of mesoporous and dense silica matrix. The nanomarkers were successfully applied for imaging of murine melanoma B16-F10 and neonatal human dermal fibroblast HDFn cell cultures by one-photon or two-photon absorption using laser scanning confocal microscopy. Great cellular uptake, low cytotoxicity and the possibility to achieve visible and NIR emission via two-photons excitation show that the nanohybrids are remarkable markers for in vitro and a potential tool for in vivo applications.

Item Type:Article
HAL Id:hal-03153161
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Other partners > Universidade Estadual Paulista - Unesp - Araraquara (BRAZIL)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Universidade de São Paulo - USP (BRAZIL)
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Deposited On:26 Feb 2021 09:29

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