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Biophysical and lipidomic biomarkers of cardiac remodeling post-myocardial infarction in humans

Samouillan, Valérie and Martinez de Lejarza Samper, Ignacio Miguel and Benitez Amaro, Aleyda and Vilades, David and Dandurand, Jany and Casas, Josefina and Jorge, Esther and de Gonzalo Calvo, David and Gallardo, Alberto and Lerma, Enrique and Guerra, Jose Maria and Carreras, Francesc and Leta, Ruben and Llorente Cortes, Vicenta Biophysical and lipidomic biomarkers of cardiac remodeling post-myocardial infarction in humans. (2020) Biomolecules, 10 (11). 1471. ISSN 2218-273X

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Official URL: https://doi.org/10.3390/biom10111471

Abstract

Few studies have analyzed the potential of biophysical parameters as markers of cardiac remodeling post-myocardial infarction (MI), particularly in human hearts. Fourier transform infrared spectroscopy (FTIR) illustrates the overall changes in proteins, nucleic acids and lipids in a single signature. The aim of this work was to define the FTIR and lipidomic pattern for human left ventricular remodeling post-MI. A total of nine explanted hearts from ischemic cardiomyopathy patients were collected. Samples from the right ventricle (RV), left ventricle (LV) and infarcted left ventricle (LV INF) were subjected to biophysical (FTIR and differential scanning calorimetry, DSC) and lipidomic (liquid chromatography–high-resolution mass spectrometry, LC–HRMS) studies. FTIR evidenced deep alterations in the myofibers, extracellular matrix proteins, and the hydric response of the LV INF compared to the RV or LV from the same subject. The lipid and esterified lipid FTIR bands were enhanced in LV INF, and both lipid indicators were tightly and positively correlated with remodeling markers such as collagen, lactate, polysaccharides, and glycogen in these samples. Lipidomic analysis revealed an increase in several species of sphingomyelin (SM), hexosylceramide (HexCer), and cholesteryl esters combined with a decrease in glycerophospholipids in the infarcted tissue. Our results validate FTIR indicators and several species of lipids as useful markers of left ventricular remodeling post-MI in humans.

Item Type:Article
Additional Information:This is an open access article under the CC BY license : https://creativecommons.org/licenses/by/4.0/. Thanks to MDPI editor. The definitive version of this document is available at: https://www.mdpi.com/2218-273X/10/11/1471
HAL Id:hal-03036091
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Other partners > Institute of Biomedical Research of Barcelona of the Spanish National Research Council - IIBB-CSIC (SPAIN)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Universitat Autònoma de Barcelona - UAB (SPAIN)
Other partners > Hospital de la Santa Creu i Sant Pau (SPAIN)
Other partners > Instituto de Salud Carlos III - ISC (SPAIN)
Other partners > Institut de Química Avançada de Catalunya - IQAC (SPAIN)
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Deposited On:02 Dec 2020 14:01

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