Merzougui, Charaf Eddine and Roblin, Pierre
and Aimar, Pierre
and Venault, Antoine and Chang, Yung and Causserand, Christel
and Bacchin, Patrice
Pearl-necklace assembly of Human serum albumin with poly (acrylic acid) polyelectrolyte explored by small angle X-ray scattering (SAXS).
(2020)
Soft Matter. ISSN 1744-683X
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(Document in English)
PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 1MB |
Official URL: https://doi.org/10.1039/D0SM01221A
Abstract
In this comprehensive study, interaction of human serum albumin (HSA) with poly(acrylic acid) (PAA) was explored using Small Angle X-ray Scattering (SAXS) combined to chromatography. Results revealed the formation of a complex between HSA macromolecules and PAA chains but solely at some specific conditions of ionic strength and pH of the medium. In fact, this binding has found to take place only at pH close to 5 and low ionic strength (0.15M). Otherwise, for higher pH and salts concentration 0.75M the HSA-PAA complex tends to dissociate completely showing the reversibility of the complexation. The assessment of the influence of the HSA/PAA molar ratio on the complex radius of gyration suggests that 4 HSA molecules could bind to each PAA 100kDa chain. Besides the Porod volume evaluation for the same range of HSA/PAA ratio confirms this assumption. Finally, an all atom SAXS modelling study using BUNCH program has been conducted to find a compatible model that fit HSA-PAA complex scattering data. This model allows to portray the HSA/PAA complex as a pear-necklace assembly with 4 HSA on the 100 kDa PAA chain.
Item Type: | Article |
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HAL Id: | hal-02960471 |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE) Other partners > R&D Center for Membrane Technology (TAIWAN) |
Laboratory name: | |
Funders: | Agence Nationale de la Recherche |
Statistics: | download |
Deposited On: | 07 Oct 2020 15:00 |
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