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Phenyllactic acid produced by Geotrichum candidum reduces Fusarium sporotrichioides and F. langsethiae rowth and T-2 Toxin Concentration

Kawtharani, Hiba and Snini, Selma and Heang, Sorphea and Bouajila, Jalloul and Taillandier, Patricia and Mathieu, Florence and Beaufort, Sandra Phenyllactic acid produced by Geotrichum candidum reduces Fusarium sporotrichioides and F. langsethiae rowth and T-2 Toxin Concentration. (2020) Toxins, 12 (4). 209. ISSN 2072-6651

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Official URL: https://doi.org/10.3390/toxins12040209

Abstract

Fusarium sporotrichioides and F. langsethiae are present in barley crops. Their toxic metabolites, mainly T-2 toxin, affect the quality and safety of raw material and final products such as beer. Therefore, it is crucial to reduce Fusarium spp. proliferation and T-2 toxin contamination during the brewing process. The addition of Geotrichum candidum has been previously demonstrated to reduce the proliferation of Fusarium spp. and the production of toxic metabolites, but the mechanism of action is still not known. Thus, this study focuses on the elucidation of the interaction mechanism between G. candidum and Fusarium spp. in order to improve this bioprocess. First, over a period of 168 h, the co-culture kinetics showed an almost 90% reduction in T-2 toxin concentration, starting at 24 h. Second, sequential cultures lead to a reduction in Fusarium growth and T-2 toxin concentration. Simultaneously, it was demonstrated that G. candidum produces phenyllactic acid (PLA) at the early stages of growth, which could potentially be responsible for the reduction in Fusarium growth and T-2 toxin concentration. To prove the PLA effect, F. sporotrichioides and F. langsethiae were cultivated in PLA supplemented medium. The expected results were achieved with 0.3 g/L of PLA. These promising results contribute to a better understanding of the bioprocess, allowing its optimization at an up-scaled industrial level.

Item Type:Article
Additional Information:Thanks to MDPI editor. This article is available at: https://www.mdpi.com/2072-6651/12/4/209/htm. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
HAL Id:hal-02640854
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
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Deposited On:05 May 2020 08:49

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