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Effects of Cyclohexane/Acetone Ratio on Bixin Extraction Yield by Accelerated Solvent Extraction Method

Rahmalia, Winda and Fabre, Jean-François and Mouloungui, Zéphirin Effects of Cyclohexane/Acetone Ratio on Bixin Extraction Yield by Accelerated Solvent Extraction Method. (2015) Procedia Chemistry, 14. 455-464. ISSN 1876-6196

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Official URL: https://doi.org/10.1016/j.proche.2015.03.061


In this study, accelerated solvent extraction (ASE) was applied to the quantitative extraction of bixin. The effects of cyclohexane/acetone ratios on bixin extraction yield were evaluated. Acetone was used in the process of pigment extraction and also played a major role in its analysis by UV-Vis spectrophotometry. Pure cis-bixin isolated by flash chromatography and characterized by Fourier Transform Infra Red spectrometry was used as a bixin standard for qualitative and quantitative analysis of annatto extracts which were obtained by accelerated solvents extraction. UV-Vis spectrophotometry analysis shows that the extraction using 100% cyclohexane gives the lowest bixin yield. This is different from the UV-Vis spectra generated with acetone extraction. The difference in percentage of acetone shows significant effects on bixin extraction yields. The use of cyclohexane:acetone (60%: 40%) solution at 50°C for 5min heating time results the highest total bixin extraction yield (48.00%) as compared to the other solvent ratios, and to the usual extraction methods using the same solvent (29.14%). High pressure liquid chromatography analysis shows that bixin extracted by this method has purity degree of 68.16%.

Item Type:Article
Additional Information:Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
HAL Id:hal-02527272
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > Tanjungpura University (INDONESIA)
Laboratory name:
Indonesian Directorate General of Higher Education - DIKTI - Tanjungpura University (INDONESIA)
Deposited On:01 Apr 2020 07:57

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