Rahmalia, Winda and Fabre, Jean-François
and Mouloungui, Zéphirin
Effects of Cyclohexane/Acetone Ratio on Bixin Extraction Yield by Accelerated Solvent Extraction Method.
(2015)
Procedia Chemistry, 14. 455-464. ISSN 1876-6196
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(Document in English)
PDF (Publisher's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 860kB |
Official URL: https://doi.org/10.1016/j.proche.2015.03.061
Abstract
In this study, accelerated solvent extraction (ASE) was applied to the quantitative extraction of bixin. The effects of cyclohexane/acetone ratios on bixin extraction yield were evaluated. Acetone was used in the process of pigment extraction and also played a major role in its analysis by UV-Vis spectrophotometry. Pure cis-bixin isolated by flash chromatography and characterized by Fourier Transform Infra Red spectrometry was used as a bixin standard for qualitative and quantitative analysis of annatto extracts which were obtained by accelerated solvents extraction. UV-Vis spectrophotometry analysis shows that the extraction using 100% cyclohexane gives the lowest bixin yield. This is different from the UV-Vis spectra generated with acetone extraction. The difference in percentage of acetone shows significant effects on bixin extraction yields. The use of cyclohexane:acetone (60%: 40%) solution at 50°C for 5min heating time results the highest total bixin extraction yield (48.00%) as compared to the other solvent ratios, and to the usual extraction methods using the same solvent (29.14%). High pressure liquid chromatography analysis shows that bixin extracted by this method has purity degree of 68.16%.
Item Type: | Article |
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Additional Information: | Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license |
HAL Id: | hal-02527272 |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE) Other partners > Tanjungpura University (INDONESIA) |
Laboratory name: | |
Funders: | Indonesian Directorate General of Higher Education - DIKTI - Tanjungpura University (INDONESIA) |
Statistics: | download |
Deposited On: | 01 Apr 2020 07:57 |
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