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Evaluation of parameters influencing plant response to carbon nanotube contamination

Liné, Clarisse. Evaluation of parameters influencing plant response to carbon nanotube contamination. PhD, Ecologie Fonctionnelle, Institut National Polytechnique de Toulouse, 2019

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Abstract

Carbon nanotubes (CNTs) are among the most used nanomaterials (NMs) thanks to their excellent physicochemical properties. All along their lifecycle, they may be spread unintentionallyor intentionally in the environment. It is thus essential to assess their behavior and potential impacts on ecosystems and particularly on crop plants. Overall, behaviour and effects of CNTs in plants are not well understood and still very controversial. In this work, we aimed to assess the influence of several parameters on plant response after exposure in a CNT-contaminated soil. We first focused on the analytical challenge of detecting CNT in biological matrices and tested several spectroscopic techniques. Then, we evaluated the response of tomato plants to two different NMs (CNTs and TiO2-NPs). Our results highlight that despite being different for several parameters (i.e. shape, size, surface chemistry), CNT exposure led to a similar response in tomato plants, in particular on the alteration of plant cell wall components. The study of different plant species (tomato, canola, maize and cucumber) exposed to DWCNT contamination highlighted different responses according to plant species, maize (monocot) being the most sensitive. Different types of CNTs are currently available. Five types of CNTs varying in diameter, functionalization and length were used to investigate their impact on canola. Canola was more sensitive to CNTs with the smallest diameters, but it was also observed that the functionalization greatly modulated the plant response. Finally, we tested the impact of a combined stress: canola plants grown in optimal growth conditions were not impacted by CNT exposure at the tested dose, while we observed that plants were more sensitive to CNTs when submitted to a concomitant heat stress.

Item Type:PhD Thesis
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Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
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Research Director:
Flahaut, Emmanuel and Larue, Camille
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Deposited On:11 Feb 2020 13:46

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