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An infrared diagnostic system to detect causal agents of grapevine trunk diseases

Levasseur-Garcia, Cécile and Malaurie, Hugo and Mailhac, Nathalie An infrared diagnostic system to detect causal agents of grapevine trunk diseases. (2016) Journal of Microbiological Methods, 131. 1-6. ISSN 0167-7012

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Official URL: https://doi.org/10.1016/j.mimet.2016.09.022

Abstract

In most vineyards worldwide, agents of grapevine trunk diseases represent a real threat for viticulture and are responsible for significant economic loss to the wine industry. The conventional microbiological isolation technique used to diagnose this disease is tedious and frequently leads to false negatives. Thus, a dire need exists for an alternative method to detect this disease. One possible way involves infrared spectroscopy, which is a rapid, nondestructive analytical tool that is commonly used for quality control of feed stuffs. In the present work, a midinfrared spectrometer was tested as a fast tool for detecting agents of grapevine trunk disease. Midinfrared spectra were collected from 70 Vitis vinifera L. cv. Cabernet-Sauvignon one year old trunk-wood samples that were infected naturally in one viticulture nursery of the south of France. The samples underwent polymerase chain reaction and morphological identification, and the results were correlated to the midinfrared spectra by using multivariate analysis to discriminate between noninfected and infected samples. Based on comparison with some control samples, the highest percentage of correct identification of fungal contamination when using the midinfrared spectroscopy method is 80%.

Item Type:Article
HAL Id:hal-02448070
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Université de Toulouse > Ecole d'Ingénieurs de Purpan - EIP (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
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Deposited On:22 Jan 2020 07:36

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