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Experimental study of fibrin/fibrin-specific molecular interactions using a sphere/plane adhesion model

Lorthois, Sylvie and Schmitz, Philippe and Anglés-Cano, Eduardo Experimental study of fibrin/fibrin-specific molecular interactions using a sphere/plane adhesion model. (2001) Journal of Colloid and Interface Science, 241 (1). 52-62. ISSN 0021-9797

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Official URL: https://doi.org/10.1006/jcis.2001.7679

Abstract

Fibrin, the biopolymer produced in the final step of the coagulation cascade, is involved in the resistance of arterial thrombi to fragmentation under shearflow. However, the nature and strength of specific interactions between fibrin monomers are unknown. Thus, the shear-induced detachment of spherical monodispersed fibrincoated latex particles in adhesive contact with a plane fibrin-coated glass surface has been experimentally studied, using an especially designed shear stress flow chamber. A complete series of experiments for measuring the shear stress necessary to release individual particles under various conditions (various number of fibrin layers involved in the adhesive contact, absence or presence of plasmin, the main physiological fibrinolytic enzyme) has been performed. The nonspecific DLVO interactions have been shown to be negligible compared to the interactions between fibrin monomers.Asimple adhesion model based on the balance of forces and torque on particles, assuming an elastic behavior of the fibrin polymer bonds, to analyze the experimental data in terms of elastic force at rupture of an elementary intermonomeric fibrin bond has been used. The results suggested that this force (of order 400 pN) is an intrinsic quantity, independent of the number of fibrin layers involved in the adhesive contact.

Item Type:Article
Additional Information:This is an open access article under the CC BY-NC-ND license : https://creativecommons.org/licenses/by-nc-nd/4.0/. Thanks to Elsevier editor. The definitive version of this document is available at: https://www.sciencedirect.com/science/article/pii/S0021979701976799?via%3Dihub
Audience (journal):International peer-reviewed journal
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Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Santé et de la Recherche Médicale - INSERM (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
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Deposited On:04 Feb 2020 07:55

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