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Bio-guided fractionation to identify Senegalia gaumeri leaf extract compounds with anthelmintic activity against Haemonchus contortus eggs and larvae

Castañeda-Ramírez, Gloria Sarahi and Torres-Acosta, Juan Felipe de Jesús and Sandoval-Castro, Carlos Alfredo and Borges-Argáez, Rocío and Cáceres-Farfán, Mirbella and Mancilla-Montelongo, Gabriela and Mathieu, Céline Bio-guided fractionation to identify Senegalia gaumeri leaf extract compounds with anthelmintic activity against Haemonchus contortus eggs and larvae. (2019) Veterinary Parasitology, 270. 13-19. ISSN 0304-4017

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Official URL: https://doi.org/10.1016/j.vetpar.2019.05.001

Abstract

Small ruminants browsing in tropical forests readily consume the foliage of Senegalia gaumeri. A S. gaumeri methanol:water extract was recently shown to have ovicidal activity against Haemonchus contortus eggs in vitro. In the present study, the fraction of a S. gaumeri methanol:water extract with ovicidal activity against H. contortus eggs and the metabolites potentially involved in this activity were identified. Bio-guided fractionation of the S. gaumeri methanol:water extract identified high ovicidal activity (80.29%, EC50 = 58.9 μg/mL) in the non-polar sub-fraction P1. Gas chromatography-mass spectrometry (GC–MS) identified several fatty acids: pentacosane (18.05%), heneicosane (18.05%), triacontane (30.94%), octacosane (18.05%), and hexanedioic acid bis-(2-ethylhexyl) ester (32.72%). Purification of the polar components of sub-fraction P1 led to the identification of p-coumaric acid as a major constituent. In egg hatch tests, 400 μg/mL p-coumaric acid resulted in an ovicidal effect of 8.7%, a larvae failing eclosion effect of 2.9%, and of the emerged larvae (88.4%), many were damaged. In conclusion, the low AH activity of p-coumaric acid against H. contortus eggs indicates that it is not solely responsible for the ovicidal activity of sub-fraction P1 but might act in synergy with other compounds in this fraction. However, p-coumaric acid showed potential anthelmintic effects against the larval stage of H. contortus.

Item Type:Article
HAL Id:hal-02381858
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Other partners > Centro de Investigación Científica de Yucatán - CICY (MEXICO)
Other partners > Consejo Nacional de Ciencia y Tecnología - CONACYT (MEXICO)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > Universidad Autónoma de Yucatán - UADY (MEXICO)
Laboratory name:
Funders:
Mexican National Council for Science and Technology - CONACYT Mexico - Catedras CONACYT
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Deposited On:26 Nov 2019 17:55

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