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Mechanistic Insights into Polyion Complex Associations

Gineste, Stéphane and Di Cola, Emanuela and Amouroux, Baptiste and Till, Ugo and Marty, Jean-Daniel and Mingotaud, Anne-Françoise and Mingotaud, Christophe and Violleau, Frédéric and Berti, Debora and Parigi, Giacomo and Luchinat, Claudio and Balor, Stéphanie and Sztucki, Michael and Lonetti, Barbara Mechanistic Insights into Polyion Complex Associations. (2018) Macromolecules, 51 (4). 1427-1440. ISSN 0024-9297

(Document in English)

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Official URL: https://doi.org/10.1021/acs.macromol.7b02391


Polyion complex (PIC) micelles formed from the electrostatic interaction between oppositely charged polymers have been studied for their promising applications in the biomedical field as drug carriers or vectors for gene delivery. In spite of their asset of possible high drug loading, their formation process remains poorly studied. In this work, we investigate the properties of a series of PICs based on poly(ethylene oxide-b-acrylic acid) (PEO–PAA)/dendrigraft poly(L-lysine) (DGL3), using PEO–PAA with different compositions and average molecular weights. For each PEO–PAA/DGL3 pair, the complexes were characterized as a function of the ratios between acid and amine moieties combining different techniques: dynamic light scattering (DLS), flow field-flow fractionation (FlFFF), small-angle X-ray scattering (SAXS), and relaxometry. The coupling of batch techniques, i.e., DLS, SAXS, and relaxometry, together with a soft separation technique like FlFFF enabled a finer analysis to elucidate subtle details of the association process and of the polydispersity of the complexes. We show that the formation of PICs is more complex than previously described. In particular, we demonstrate that PICs with stoichiometry 1:1 may form at low ratios provided that the acidic block is long enough to neutralize the cationic dendrigraft with few polymer chains. Moreover, in such conditions, PICs with stoichiometry 1:1 often coexist with free dendritic polymers and other associated complex species.

Item Type:Article
HAL Id:hal-02171171
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Ecole d'Ingénieurs de Purpan - EIP (FRANCE)
Other partners > European Synchrotron Radiation Facility - ESRF (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Università degli Studi di Milano (ITALY)
Other partners > Organisation Européenne pour la Recherche Nucléaire - CERN (SWITZERLAND)
Other partners > Università degli Studi di Firenze (ITALY)
Laboratory name:
Deposited On:20 Nov 2019 09:55

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