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Rapid characterization of aquatic hyphomycetes by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Cornut, Julien and De Respinis, Sophie and Tonolla, Mauro and Petrini, Orlando and Bärlocher, Felix and Chauvet, Eric and Bruder, Andreas Rapid characterization of aquatic hyphomycetes by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. (2019) Mycologia, 111 (1). 177-189. ISSN 0027-5514

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Official URL: https://doi.org/10.1080/00275514.2018.1528129

Abstract

Protein fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spec-trometry (MALDI-TOF MS) is a rapid, reliable, and economical method to characterize isolates of terrestrial fungi and other microorganisms. The objective of our study was to evaluate the suitability of MALDI-TOF MS for the identification of aquatic hyphomycetes, a polyphyletic group of fungi that play crucial roles in stream ecosystems. To this end, we used 34 isolates of 21 aquatic hyphomycete species whose identity was confirmed by spore morphology and internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) nuc rDNA sequencing. We tested the efficiency of three protein extraction methods, including chemical and mechanical treatments using 13 different protocols, with the objective of producing high-quality MALDI-TOF mass spectra. In addition to extraction protocols, mycelium age was identified as a key parameter affecting protein extraction efficiency. The dendrogram based on mass-spectrum similarity indicated good and relevant taxonomic discrimination; the tree structure was comparable to that of the phylogram based on ITS sequences. Consequently, MALDI-TOF MS could reliably identify the isolates studied and provided greater taxonomic accuracy than classical morphological methods. MALDI-TOF MS seems suited for rapid characterization and identification of aquatic hyphomycete species.

Item Type:Article
HAL Id:hal-02359264
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université de Genève (SWITZERLAND)
Other partners > Mount Allison University - MTA (CANADA)
Other partners > POLE Pharma Consulting (SWITZERLAND)
Other partners > University of Applied Sciences and Arts of Southern Switzerland - SUPSI (SWITZERLAND)
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Deposited On:15 Nov 2019 12:54

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