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Luminescent biomimetic citrate-coated europium-doped carbonated apatite nanoparticles for use in bioimaging: physico-chemistry and cytocompatibility

Gómez-Morales, Jaime and Verdugo-Escamilla, Cristóbal and Fernández-Penas, Raquel and Parra-Milla, Carmen María and Drouet, Christophe and Bosc, Françoise and Oltolina, Francesca and Prat, Maria and Fernández-Sánchez, Jorge Fernando Luminescent biomimetic citrate-coated europium-doped carbonated apatite nanoparticles for use in bioimaging: physico-chemistry and cytocompatibility. (2018) RSC Advances, 8 (5). 2385-2397. ISSN 2046-2069

(Document in English)

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Official URL: https://doi.org/10.1039/C7RA12536D


Nanomedicine covers the application of nanotechnologies in medicine. Of particular interest is the setup of highly-cytocompatible nanoparticles for use as drug carriers and/or for medical imaging. In this context, luminescent nanoparticles are appealing nanodevices with great potential for imaging of tumor or other targetable cells, and several strategies are under investigation. Biomimetic apatite nanoparticles represent candidates of choice in nanomedicine due to their high intrinsic biocompatibility and to the highly accommodative properties of the apatite structure, allowing many ionic substitutions. In this work, the preparation of biomimetic (bone-like) citrate-coated carbonated apatite nanoparticles doped with europium ions is explored using the citrate-based thermal decomplexing approach. The technique allows the preparation of the single apatitic phase with nanosized dimensions only at Eu3+ doping concentrations ≤0.01 M at some timepoints. The presence of the citrate coating on the particle surface (as found in bone nanoapatites) and Eu3+ substituting Ca2+ is beneficial for the preparation of stable suspensions at physiological pH, as witnessed by the ζ-potential versus pH characterizations. The sensitized luminescence features of the solid particles, as a function of the Eu3+ doping concentrations and the maturation times, have been thoroughly investigated, while those of particles in suspensions have been investigated at different pHs, ionic strengths and temperatures. Their cytocompatibility is illustrated in vitro on two selected cell types, the GTL-16 human carcinoma cells and the m17.ASC murine mesenchymal stem cells. This contribution shows the potentiality of the thermal decomplexing method for the setup of luminescent biomimetic apatite nanoprobes with controlled features for use in bioimaging.

Item Type:Article
Additional Information:This is an open access article under the CC BY-NC Thanks to The Royal Society of Chemistry editor. The definitive version of this document is available at: https://pubs.rsc.org/en/content/articlelanding/2018/RA/C7RA12536D#!divAbstract
HAL Id:hal-02335630
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Universidad de Granada - UGR (SPAIN)
Other partners > Università degli Studi del Piemonte Orientale-Amedeo Avogadro (ITALY)
Laboratory name:
Deposited On:28 Oct 2019 12:49

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