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A zwitterionic interpenetrating network for improving the blood compatibility of polypropylene membranes applied to leukodepletion

Lien, Cheng-Chi and Chen, Po-Ju and Venault, Antoine and Tang, Shuo-Hsi and Fu, Ying and Dizon, Gian Vincent and Aimar, Pierre and Chang, Yung A zwitterionic interpenetrating network for improving the blood compatibility of polypropylene membranes applied to leukodepletion. (2019) Journal of Membrane Science, 584. 148-160. ISSN 0376-7388

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Official URL: https://doi.org/10.1016/j.memsci.2019.04.056


Although widely used in blood-contacting devices, polypropylene (PP) membranes are prone to biofouling by plasma proteins and blood cells. The present study explores the effect of a surface zwitterionization process on the improvement of the biofouling resistance of PP membranes for leukocyte reduction filters. The modification strategy consists in forming an interpenetrating network of poly(glycidyl methacrylate-co-sulfobetaine methacrylate) (poly(GMA-co-SBMA) around the fibers of coated PP membranes, using a cross-linking agent: ethylenediamine (EDA). It is shown that with EDA, a range of poly(GMA-co-SBMA) concentration (1–5 mg/mL) leads to a 0°-water contact angle and high hydration of the networks without affecting the intrinsic porous structure of the material. Besides, the related membranes show excellent resistance to biofouling by Escherichia coli, fibrinogen, leukocytes, erythrocytes, thrombocytes and cells from whole blood with reductions in adsorption of 97%, 86%, 90%, 95%, 97% and 91%, respectively, compared to unmodified PP. Used in whole blood filtration, it is demonstrated that in the best conditions (5 mg/mL copolymer, with EDA), leukocytes can be efficiently removed (>99.99%) without altering the erythrocytes concentration in the permeate, and that leukodepletion is more efficient than that measured with a commercial hydrophilic PP blood filter (about 50% retention). Physical retention of leukocytes is only efficient if the membrane material is anti-biofouling, and so, does not interact with other blood components able to trigger leukocyte attachment/deformation.

Item Type:Article
HAL Id:hal-02292034
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Chung Yuan Christian University - CYCU (TAIWAN)
Laboratory name:
Ministry of Science and Technology, Taiwan
Deposited On:19 Sep 2019 11:16

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