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Response surface methodology to optimise Accelerated Solvent Extraction of steviol glycosides from Stevia rebaudiana Bertoni leaves

Jentzer, Jean-Baptiste and Alignan, Marion and Vaca-Garcia, Carlos and Rigal, Luc and Vilarem, Gérard Response surface methodology to optimise Accelerated Solvent Extraction of steviol glycosides from Stevia rebaudiana Bertoni leaves. (2015) Food Chemistry, 166. 561-567. ISSN 0308-8146

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Official URL: https://doi.org/10.1016/j.foodchem.2014.06.078

Abstract

Following the approval of steviol glycosides as a food additive in Europe in December 2011, large-scale stevia cultivation will have to be developed within the EU. Thus there is a need to increase the efficiency of stevia evaluation through germplasm enhancement and agronomic improvement programs. To address the need for faster and reproducible sample throughput, conditions for automated extraction of dried stevia leaves using Accelerated Solvent Extraction were optimised. A response surface methodology was used to investigate the influence of three factors: extraction temperature, static time and cycle number on the stevioside and rebaudioside A extraction yields. The model showed that all the factors had an individual influence on the yield. Optimum extraction conditions were set at 100°C, 4min and 1 cycle, which yielded 91.8%±3.4% of total extractable steviol glycosides analysed. An additional optimisation was achieved by reducing the grind size of the leaves giving a final yield of 100.8%±3.3%.

Item Type:Article
HAL Id:hal-02063665
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > King Abdulaziz University - KAU (SAUDI ARABIA)
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Deposited On:11 Mar 2019 12:24

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