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Influence of microalgae cell wall characteristics on protein extractability and determination of nitrogen-to-protein conversion factors

Safi, Carl and Charton, Michael and Pignolet, Olivier and Silvestre, Françoise and Vaca-Garcia, Carlos and Pontalier, Pierre-Yves Influence of microalgae cell wall characteristics on protein extractability and determination of nitrogen-to-protein conversion factors. (2013) Journal of Applied Psychology, 25 (2). 523-529. ISSN 0921-8971

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Official URL: https://doi.org/10.1007/s10811-012-9886-1

Abstract

Additional evidence about the influence of the cell wall physical and chemical characteristics on protein extractability was determined by calculating the conversion factors of five different microalgae known to have different cell wall composition, and their protein extracts. The conversion factors obtained for crude rigid cell walled Chlorella vulgaris, Nannochloropsis oculata and Haematococcus pluvialis were 6.35, 6.28 and 6.25, respectively, but for their protein extracts the values were lower with 5.96, 5.86 and 5.63. On the other hand, conversion factor obtained for fragile cell walled microalgae Porphyridium cruentum and Athrospira platensis was 6.35 for the former and 6.27 for the latter, with no significant difference for their protein extract with 6.34 for the former and 6.21 for the latter. In addition, the highest hydro-soluble protein percentage recovered from total protein was for P. cruentum 80.3 % and A. platensis 69.5 % but lower for C. vulgaris with 43.3 %, N. oculata with 33.3 % and H. pluvialis with 27.5 %. The study spotted the light on the influence of the cell wall on evaluating the conversion factor and protein extractability. In addition, it showed the necessity of finding the conversion factor every time accurate protein quantification is required, and proved that there is not a universal conversion factor that can be recommended.

Item Type:Article
HAL Id:hal-02064796
Audience (journal):International peer-reviewed journal
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Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
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Deposited On:12 Mar 2019 10:02

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