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A two-stage ultrafiltration process for separating multiple components of Tetraselmis suecica after cell disruption

Safi, Carl and Liu, Dylan Z. and Yap, Benjamin H. J. and Martin, Gregory J. O. and Vaca-Garcia, Carlos and Pontalier, Pierre-Yves A two-stage ultrafiltration process for separating multiple components of Tetraselmis suecica after cell disruption. (2014) Journal of Applied Psychology, 26 (6). 2379-2387. ISSN 0921-8971

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Official URL: https://doi.org/10.1007/s10811-014-0271-0


A two-stage ultrafiltration process was applied to the aqueous phase of Tetraselmis suecica after breaking its cell wall by high-pressure homogenization. Microscopic observation revealed that the cells were completely disrupted from 600 bar and cell fragmentation of the cells was also noticeable after 800 bar. In addition, the highest concentration of all the molecules of interest in the aqueous phase was observed at 1,000 bar and a temperature of 46 °C while preserving the integrity of the molecules of interest in the downstream process. After centrifugation, the aqueous phase was submitted to ultrafiltration through two consecutive membranes of different molecular weight cutoffs. Complete retention of starch was possible with a 100-kDa membrane and separation of sugars from proteins with a 10-kDa membrane on the remaining mixture. After testing the process with model solutions, the transmembrane pressure selected was 2.07 bar, which succeeded in retaining starch and pigments during the first part of the process, and proteins during the second part. A linear correlation between the permeate flux rate and the pressure was observed in both parts of the process.

Item Type:Article
HAL Id:hal-02079755
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
Other partners > King Abdulaziz University - KAU (SAUDI ARABIA)
Other partners > University of Melbourne (AUSTRALIA)
Laboratory name:
Deposited On:26 Mar 2019 10:37

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