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Metabolisation of eutypine by plant tissues: an HPLC determination

Afifi, Maha and Monje, Marie-Carmen and Legrand, Valérie and Roustan, Jean-Paul and Nepveu, Françoise Metabolisation of eutypine by plant tissues: an HPLC determination. (2004) Analytica Chimica Acta, 513 (1). 21-27. ISSN 0003-2670

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Official URL: https://doi.org/10.1016/j.aca.2003.10.018

Abstract

Eutypine, 4-hydroxy-3-(3-methyl-3-butene-1-ynyl) benzaldehyde, is a toxin produced by Eutypa lata, the causal agent of eutypa dieback of grapevine. The tolerance of some grapevine cultivars to the disease has been ascribed to the potential reduction of eutypine into its corresponding non-toxic alcohol, eutypinol. In the present study, eutypine biotransformation in different tissues of grapevine was investigated by HPLC and LC–MS. Grape callus tissues were able to biotransform eutypine into eutypinol within the first 3 h of culture. The grape plantlets cultured in vitro can also transform eutypine into eutypinol. Grape plantlet leaves do not have any effect on the uptake of eutypine, which goes through the tissues following a concentration gradient. Results revealed that the toxicity of eutypine in grape tissues is an active process showing that eutypinol is rapidly metabolised into other compounds. The use of micro-cuttings and in vitro plants showed that eutypine strongly accumulates in the bottom part of the diseased plant stems.

Item Type:Article
HAL Id:hal-02073889
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
French research institutions > Institut de Recherche pour le Développement - IRD (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
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Deposited On:20 Mar 2019 11:03

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