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Improvement of a fed-batch process for high level xylanase production by a Bacillus strain

Schneider, Gerhard and Strehaiano, Pierre and Taillandier, Patricia Improvement of a fed-batch process for high level xylanase production by a Bacillus strain. (2001) Journal of Chemical Technology & Biotechnology, 76 (5). 456-460. ISSN 0268-2575

(Document in English)

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Official URL: https://doi.org/10.1002/jctb.415


In this paper, the improvement of a fed-batch fermentation from the point of view of an industrial xylanase production process is described. The Bacillus strain chosen for this study is able to produce high quantities of a xylanase that is suitable to be used as bleach boost agent in chlorine-free bleaching sequences of paper pulp. It was found that xylo-oligosaccharides hydrolysis products from xylan by xylanase action were indispensable for induction of the enzyme synthesis, but that their presence in quantities of only 0.1g dm^-3 xylose equivalents led to catabolite repression. A substrate-limited fed-batch process, that is the most adapted, was furthermore improved with regard to nutrient requirement of the microorganism, especially the nitrogen source. A process with constant supply of a culture medium containing xylan, peptone and mineral nitrogen was able to produce 20240nkatcm^-3 with a productivity of 910nkatcm^-3 h^-1, which places the process among the best ever reported.

Item Type:Article
HAL Id:hal-02135100
Audience (journal):International peer-reviewed journal
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Laboratory name:
European Union
Deposited On:04 Apr 2019 07:39

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