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Analysis of the effectiveness of a cereal milling by product monocomponent medium for the low cost production of Bacillus thuringiensis

Rahbany, Gihane and Salameh, Dominique and Brandam, Cédric and Lteif, Roger Analysis of the effectiveness of a cereal milling by product monocomponent medium for the low cost production of Bacillus thuringiensis. (2014) In: 16th European Congress on Biotechnology, 13 July 2014 - 16 July 2014 (Edinburgh, United Kingdom).

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Official URL: https://doi.org/10.1016/j.nbt.2014.05.1967


Bacillus thuringiensis is a facultative anaerobe, gram positive, spore forming bacterium. The biotechnological importance of this bacterium resides in its ability to produce, during sporulation, crystal proteins known as delta-endotoxins which express specific insecticidal activity. At industrial scale, the culture media represents an important part of B. thuringiensis based biopesticides production cost. According to the literature, different agro-industrial residues and byproducts were used as sources of proteins in order to reduce the cost of B. thuringiensis culture medium, but carbohydrates (glucose, starch or molasses) and/or mineral sources were added. In this work, a cereal milling by-product (CMB) as a monocomponent medium was investigated and compared to synthetic mediums in terms of delta-endotoxin yield and productivity in submerged fermentation of different strains of B. thuringiensis. The CMB was shown efficient to be used as a complete substrate (source of proteins, carbohydrates and minerals) for B. thuringiensis production. The optimal CMB ratio in the culture medium was found to be 6% in shake flasks experiments. The consumption of the CMB sugars by the bacteria was analyzed. Production of the bio-insecticide in lab-bioreactor in controlled conditions was equally performed to give basic elements for extrapolation in industrial conditions.

Item Type:Conference or Workshop Item (Other)
HAL Id:hal-02042384
Audience (journal):Special issue journal
Audience (conference):International conference without published proceedings
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Other partners > Université Saint-Joseph de Beyrouth - USJ (LEBANON)
Laboratory name:
Deposited On:20 Feb 2019 12:15

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