Chan, Sitha and Kanchanatawee, Sunthorn and Jantama, Sirima Suvarnakuta and Jantama, Kaemwich and Joannis-Cassan, Claire
and Taillandier, Patricia
Using a combined oxygen-supply and substrate-feeding strategy to improve 2,3-butanediol production by metabolically engineered Klebsiella oxytoca KMS005.
(2017)
Journal of Chemical Technology & Biotechnology, 93 (2). 600-608. ISSN 0268-2575
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(Document in English)
PDF (Author's version) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader 1MB |
Official URL: https://doi.org/10.1002/jctb.5409
Abstract
Abstract BACKGROUND There is much demand for and extensive application for 2,3-butanediol (2,3-BD) in various fields, and micro-aerobic and substrate-feeding conditions greatly affect microbial growth and production. The theoretical maximum of 2,3-BD fermentative yield has rarely been reported. Therefore, our study aimed to develop an efficient combined oxygen-supply and substrate-feeding strategy to improve 2,3-BD production yield in metabolically engineered Klebsiella oxytoca KMS005. RESULTS The optimized oxygen consumption for 2,3-BD production by strain KMS005 was demonstrated at 9.2 g for 1 L working volume corresponding to KLa of 25.2 h−1. During fed-batch, a glucose feeding rate of 2 g h−1 starting at the end of the growth phase for 48 h followed by a final batch phase of 40 h was found likely to be satisfactory for 2,3-BD production by the strain KMS005. A final 2,3-BD concentration was obtained at 74.7 g L−1 with few by-products formation. A theoretical maximum of 2,3-BD production yield of 0.5 g g−1 substrate used was also approached. CONCLUSION Our oxygen-supply strategy with the specific feeding pattern developed in this study allowed the highest fermentative production yield of 2,3-BD ever reported. The KMS005 strain may be used as a biocatalyst for cost-effective 2,3-BD production from renewable substrates. In addition, the outcome might bring a message for further developments of simple fed-batch fermentation under micro-aeration conditions into larger scales for 2,3-BD production by K. oxytoca KMS005 or even other microorganisms.
Item Type: | Article |
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HAL Id: | hal-01990393 |
Audience (journal): | International peer-reviewed journal |
Uncontrolled Keywords: | |
Institution: | French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE) Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) Other partners > Ubon Ratchathani University - UBU (THAILAND) Université de Toulouse > Université Toulouse III - Paul Sabatier - UT3 (FRANCE) Other partners > Suranaree University of Technology - SUT (THAILAND) |
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Deposited On: | 23 Jan 2019 08:41 |
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