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DNA Metabarcoding to quantify the response of insect diversity to mountain forest die-offs in the French Pyrenees

Sire, Lucas and Rougerie, Rodolphe and Larrieu, Laurent and Bézier, Annie and Courtial, Béatrice and Bouget, Christophe and Herniou, Elisabeth and Lopez Vaamonde, Carlos DNA Metabarcoding to quantify the response of insect diversity to mountain forest die-offs in the French Pyrenees. (2018) In: International Conference on Ecological Sciences (Sfécologie 2018), 22 October 2018 - 25 October 2018 (Rennes, France).

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Official URL: https://prodinra.inra.fr/record/452081

Abstract

Mountain forests suffer from an increase in frequency and severity of summer droughts and infestations of pathogens and insects. Those factors are causing high mortality of some keystone tree species (forest die-offs). Yet, how tree diebacks and associated changes in forest composition will affect local diversity and ecosystem functions remains unknown. Here, we aim at quantifying the impact of climate-induced forest decline on biodiversity by measuring changes in taxonomic structure of invertebrate communities along gradients of silver fir (Abies alba) dieback in the French Pyrenees. We use DNA metabarcoding to analyse 224 samples of Malaise traps placed on 56 silver fir dominated plots in the French Pyrenees from May to September 2017. Samples were sequenced using Illumina MiSeq and analysed using the DAMe twin-tagging pipeline approach. We conducted taxonomic assignment against reference DNA barcode libraries to streamli ne identification and recover biological information for ecological analysis. We discuss the results of our metabarcoding analysis and the utility of our approach to conduct biomonitoring across a large geographical scale.

Item Type:Conference or Workshop Item (Other)
ProdINRA Id:452081
Audience (conference):International conference proceedings
Uncontrolled Keywords:
Institution:French research institutions > Centre National de la Recherche Scientifique - CNRS (FRANCE)
Université de Toulouse > Ecole d'Ingénieurs de Purpan - EIP (FRANCE)
Université de Toulouse > Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
French research institutions > Institut National de la Recherche Agronomique - INRA (FRANCE)
French research institutions > Institut national de Recherche en Sciences et Technologies pour l'Environnement et l'Agriculture - IRSTEA (FRANCE)
Other partners > Museum National d'Histoire Naturelle - MNHN (FRANCE)
Other partners > Université de Tours (FRANCE)
Laboratory name:
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Deposited On:31 Mar 2020 11:10

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